abstract：:The basic fibroblast growth factor (FGF) is a potent mitogen that has vascular endothelium as one of its principle target cells. Recent work has provided both the complete amino acid sequence of basic FGF and the nucleotide sequence of the genes for both human and bovine basic FGF. Although capillary endothelial cells...

journal_title：Journal of cellular physiology

authors： Hanneken A,Lutty GA,McLeod DS,Robey F,Harvey AK,Hjelmeland LM

更新日期：1989-01-01 00:00:00

abstract：:Rat pheochromocytoma PC12 cells respond to the binding of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) by extending neurites in a manner resembling sympathetic neurons. This response requires cell attachment to an appropriate substratum (Fujii et al., J. Neurosci., 2:1157, 1982); attachment fact...

journal_title：Journal of cellular physiology

authors： Schwarz MA,Brown PJ,Eveleth DD,Bradshaw RA

更新日期：1989-01-01 00:00:00

abstract：:Proliferation of human skin fibroblasts was stimulated significantly by the presence of L-ascorbic acid 2-phosphate (Asc 2-P). The presence of Asc 2-P (0.1-1.0 mM) in the culture medium for 3 weeks enhanced the relative rate of collagen synthesis to total protein synthesis 2-fold as well as cell growth 4-fold. Coexist...

journal_title：Journal of cellular physiology

authors： Hata R,Senoo H

更新日期：1989-01-01 00:00:00

abstract：:The F-40 cell line, a stable variant of LM fibroblasts selected for its resistance to polyethylene glycol (PEG)-induced fusion (Roos and Davidson: Somatic Cell and Molecular Genetics 6:381-391, 1980), has a decreased capacity to internalize fluid-phase markers and nonspecifically surface-bound macromolecules. It is no...

journal_title：Journal of cellular physiology

authors： Ryser HJ,Li W,Mandel R,Shen WC

更新日期：1988-12-01 00:00:00

abstract：:Several organic cations are actively transported by proximal renal tubules by mediated processes across both the apical and basolateral cell membranes. In order to evaluate this transport system in a cultured renal epithelium, uptake of 3H-tetraethylammonium (TEA) across the apical membrane was measured in LLCPK1 cell...

journal_title：Journal of cellular physiology

authors： McKinney TD,DeLeon C,Speeg KV Jr

更新日期：1988-12-01 00:00:00

abstract：:Adhesion of human monocytes and granulocytes to vascular endothelium plays an important role in migration of these cells to inflammatory sites in tissues. A family of three human leukocyte heterodimeric surface molecules named Mo1, LFA-1, and p150,95 (LeuM5) has been shown to mediate leukocyte adhesion to confluent mo...

journal_title：Journal of cellular physiology

authors： Arnaout MA,Lanier LL,Faller DV

更新日期：1988-11-01 00:00:00

abstract：:Epidermal growth factor (EGF) is a small protein that acts as a mitogen for various epidermal, epithelial, and fibroblastic cells that bear specific EGF receptors. The molecule that binds EGF is a 175-kD transmembrane protein, with an extracellular ligand binding domain and an intracellular domain that possesses tyros...

journal_title：Journal of cellular physiology

authors： Collins MK,Downward J,Miyajima A,Maruyama K,Arai K,Mulligan RC

更新日期：1988-11-01 00:00:00

abstract：:Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by...

journal_title：Journal of cellular physiology

authors： Millar JB,Rozengurt E

更新日期：1988-11-01 00:00:00

abstract：:Control of the terminal event in cellular differentiation is an important normal regulatory process, and the expression of defects in the control of this process has been implicated in the pathogenesis of cancer. To determine if tumor necrosis factor (TNF), which is an important biological response modifier, can inhib...

journal_title：Journal of cellular physiology

authors： Filipak M,Sparks RL,Tzen CY,Scott RE

更新日期：1988-11-01 00:00:00

abstract：:The effects of the polyamines putrescine (PUT), spermidine (SPD), and spermidine (SPM) on the secretion of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) were evaluated using cultured bovine aortic endothelial cells. All three polyamines enhanced PA secretion in a time- and dose-dependent manner,...

journal_title：Journal of cellular physiology

authors： Kuo BS,Korner G,Dryjski M,Bjornsson TD

更新日期：1988-10-01 00:00:00

abstract：:Incubation of cultured bovine pulmonary artery endothelial cells with 200 microM of 3-isobutyl-1-methylxanthine (IBMX) for 24 hr produced a five- to tenfold increase in cellular angiotensin converting enzyme activity (ACE) above that of untreated control cells. A lesser increase was observed in medium ACE. Other methy...

journal_title：Journal of cellular physiology

authors： Dasarathy Y,Fanburg BL

更新日期：1988-10-01 00:00:00

abstract：:We have previously observed major differences in the phosphorylation of membrane proteins in sparse, proliferating versus confluent, quiescent pig aortic endothelial cells (EC) (Kazlauskas and DiCorleto, 1987). In the present study we examined whether EC growth state can influence the activity of a specific phosphoryl...

journal_title：Journal of cellular physiology

authors： Uratsuji Y,DiCorleto PE

更新日期：1988-09-01 00:00:00

abstract：:Hepatic sinusoidal Ito cells have the capacity to produce interstitial collagen types I and III as well as other matrix proteins and may be involved in hepatic fibrogenesis. Transforming growth factor beta (TGF beta) responsiveness was evaluated during in vitro cell culture, since increasing evidence suggests that thi...

journal_title：Journal of cellular physiology

authors： Davis BH

更新日期：1988-09-01 00:00:00

abstract：:We have investigated the kinetics of fluid-phase endocytosis and diacytosis in confluent monolayers of human fibroblasts by comparing the behavior of three markers that have been previously used to study this process: [14C]sucrose, 125I-labeled polyvinylpyrrolidone ([125I]PVP), and Lucifer Yellow. Three distinct kinet...

journal_title：Journal of cellular physiology

authors： McKinley DN,Wiley HS

更新日期：1988-09-01 00:00:00

abstract：:The calcium-sensitive fluorescent indicator fura-2 and a microscope equipped for rapidly changing excitation wavelengths were used to look at the effects of growth factors on cytosolic free calcium ([Ca2+]i) in NRK-49F cells. In these cells bradykinin induced a rapid increase in [Ca2+]i, which generally decayed to nea...

journal_title：Journal of cellular physiology

authors： Marks PW,Kruskal BA,Maxfield FR

更新日期：1988-09-01 00:00:00

abstract：:We examined the role of superoxide in the increase in intracellular pH (pHi) of human histiocytic leukemia U937 cells treated with 4 beta-phorbol-12,13-didecanoate (4 beta-PDD) or serum. 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated h...

journal_title：Journal of cellular physiology

authors： Shibanuma M,Kuroki T,Nose K

更新日期：1988-08-01 00:00:00

abstract：:The differentiation of human keratinocytes in most culture systems is incomplete; e.g., lamellar bodies, the characteristic lipid-delivery organelles of epidermis, are not present. Moreover, their lipid profile does not reflect the distinctive composition found in cornifying epidermis. In contrast, keratinocytes that ...

journal_title：Journal of cellular physiology

authors： Williams ML,Brown BE,Monger DJ,Grayson S,Elias PM

更新日期：1988-07-01 00:00:00

abstract：:We have reported previously that the production of a tumor cell factor that stimulates synthesis of fibroblast collagenase is influenced by a fibroblast-deposited matrix component, possibly heparan sulfate-proteoglycan. In this study, binding sites for heparin and heparan sulfate on mouse B-16 melanoma cells have been...

journal_title：Journal of cellular physiology

authors： Biswas C

更新日期：1988-07-01 00:00:00

abstract：:Pleiotropic human KB cell mutants, selected for resistance to a conjugate of epidermal growth factor with Pseudomonas exotoxin (PE-EGF), were characterized genetically. These mutants have a pleiotropic phenotype, which includes reduced number of EGF receptors and reduced growth rate. Hybrid cells between HeLa D98 and ...

journal_title：Journal of cellular physiology

authors： Amano F,Pastan I,Gottesman MM

更新日期：1988-06-01 00:00:00

abstract：:Comparative studies with rat transferrin (rTf) and asialotransferrin (asialo-rTf) were performed on suspended adult rat hepatocytes with the aim of elucidating the mechanism of enhanced hepatic Fe acquisition from asialo-rTf observed previously in vivo. At low ligand concentrations (0.05-20 micrograms/ml), the cells b...

journal_title：Journal of cellular physiology

authors： Rudolph JR,Regoeczi E

更新日期：1988-06-01 00:00:00

abstract：:The synthesis and turnover of metabolically labeled proteoglycans from medium, cell layer, and substratum-associated compartments were characterized in four cell lines of fibroblastic origin, including a fibrosarcoma line, and in the murine melanoma cell type, B16.F10. Substantial differences were apparent between the...

journal_title：Journal of cellular physiology

authors： Piepkorn M,Hovingh P,Linker A

更新日期：1988-05-01 00:00:00

abstract：:The regulation of low-density lipoprotein (LDL) receptor activity in the human hepatoma cell line Hep-G2 by serum components was examined. Incubation of dense monolayers of Hep-G2 cells with fresh medium containing 10% fetal calf serum (FM) produced a time-dependent increase in LDL receptor activity. Uptake and degrad...

journal_title：Journal of cellular physiology

authors： Ellsworth JL,Brown C,Cooper AD

更新日期：1988-05-01 00:00:00

abstract：:We have studied the transcriptional activity, steady-state mRNA levels, and steady-state protein levels of the c-myc and transferrin receptor (TfR) genes in murine M1 myeloid leukemia cells arrested in G1 phase of the cell cycle by different methods. When cells are growth-arrested by density inhibition, a technique th...

journal_title：Journal of cellular physiology

authors： Neckers LM,Tsuda H,Weiss E,Pluznik DH

更新日期：1988-05-01 00:00:00

abstract：:Using a recently described technique of electron spin resonance spectroscopy, we determined the rate of transbilayer mobility (flip) of the four major simian-erythrocyte phospholipids in the erythrocyte membrane after infection by Plasmodium knowlesi. The development of the malarial parasite induces a very large incre...

journal_title：Journal of cellular physiology

authors： Beaumelle BD,Vial HJ,Bienvenüe A

更新日期：1988-04-01 00:00:00

abstract：:The amino acid pools in Chinese hamster lung V79 cells were measured as a function of time during hyperthermic exposure at 40.5 degrees and 45.0 degrees C. Sixteen of the 20 protein amino acids were present in sufficient quantity to measure accurately. The total amino acid pool and all individual amino acids, except g...

journal_title：Journal of cellular physiology

authors： Goh CJ,Dumbroff EB,Lepock JR

更新日期：1988-04-01 00:00:00

abstract：:The interaction between dietary lectins, especially wheat germ agglutinin (WGA), and intestinal cells has been implicated in the pathogenesis of celiac disease. The present study was undertaken to investigate the immediate effects following such an interaction. Direct WGA-stimulation of Intestine 407 cells leads to an...

journal_title：Journal of cellular physiology

authors： Sjölander A

更新日期：1988-03-01 00:00:00

abstract：:The objective of this study was to establish whether human recombinant tumor necrosis factor (TNF) can significantly stimulate the proliferation of some tumor cells. Treatment with TNF had little or no effect on the growth of human tumor cells and murine NIH/3T3 cells cultured in medium with high serum concentration. ...

journal_title：Journal of cellular physiology

authors： Kirstein M,Baglioni C

更新日期：1988-03-01 00:00:00

abstract：:Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cell...

journal_title：Journal of cellular physiology

authors： Iwamoto Y,Graf J,Sasaki M,Kleinman HK,Greatorex DR,Martin GR,Robey FA,Yamada Y

更新日期：1988-02-01 00:00:00

abstract：:A cloned human hepatoma cell line (Li-7A), possessing epidermal growth factor (EGF) receptors numbering in the range of 10-20 pmol/10(6) cells, was inhibited in its growth by EGF as well as an antagonist monoclonal antibody (MoAb) to the EGF receptor. The mode of action of the two ligands of EGF receptors appeared to ...

journal_title：Journal of cellular physiology

authors： Knowles AF

更新日期：1988-01-01 00:00:00

abstract：:In spite of the apparent depletion of mast cells in tissues of mutant mice of W/Wv genotype, cells with many features of mast cells do develop when bone marrow cells of W/Wv mice are cultured in the presence of pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM). In order to resolve this discrepancy a...

journal_title：Journal of cellular physiology

authors： Fujita J,Nakayama H,Onoue H,Kanakura Y,Nakano T,Asai H,Takeda S,Honjo T,Kitamura Y

更新日期：1988-01-01 00:00:00

abstract：:Fluorescence anisotropy measurements are widely used as sensitive indicators of cell membrane fluidity. 1-[4-(trimethylamino)phenyl]-6-phenyl hexa-1,3,5-triene (TMA-DPH) is a cationic fluorescent aromatic hydrocarbon that anchors at the lipid-water interface of membrane lipid bilayers. Its uptake into porcine pulmonar...

journal_title：Journal of cellular physiology

authors： Sheridan NP,Block ER

更新日期：1988-01-01 00:00:00

abstract：:Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in cultu...

journal_title：Journal of cellular physiology

authors： Rearick JI,Hesterberg TW,Jetten AM

更新日期：1987-12-01 00:00:00

abstract：:A temperature-sensitive mutant with a defect in glycoprotein synthesis and a cell cycle (G1)-specific arrest at the nonpermissive temperature (Tenner et al., J. Cell. Physiol., 90:145-160, 1977; Tenner and Scheffler, J. Cell. Physiol., 98:251-266, 1979) was investigated further after a human epidermal growth factor (E...

journal_title：Journal of cellular physiology

authors： Feige JJ,Scheffler IE

更新日期：1987-12-01 00:00:00

abstract：:In the presence of lovastatin (mevinolin), an inhibitor of endogenous mevalonate synthesis, C1300 murine neuroblastoma cells incorporated (2-14C)mevalonate into several discrete polypeptides that were separable by SDS-PAGE. The electrophoretic pattern of the labeled proteins did not vary substantially when cells were ...

journal_title：Journal of cellular physiology

authors： Maltese WA,Sheridan KM

更新日期：1987-12-01 00:00:00

abstract：:Hyaluronate degradation was examined in cultures of vascular wall cells (bovine aortic endothelial cells, rat aortic smooth muscle cells) and in nonvascular cells (chick embryo fibroblasts). The three cell types examined all produced hyaluronidase activity in culture which had a strict acidic pH requirement for activi...

journal_title：Journal of cellular physiology

authors： McGuire PG,Castellot JJ Jr,Orkin RW

更新日期：1987-11-01 00:00:00

abstract：:The oxygen dependence of hepatic cellular respiration was studied by employing simultaneous organ spectrophotometry of cytochromes and hemoglobin, the latter used as an intrasinusoidal optical oxygen probe. The Km of cytochrome aa3 for oxygen was found to be 6.8 microM in the isolated perfused liver and 0.3 microM in ...

journal_title：Journal of cellular physiology

authors： Kekonen EM,Jauhonen VP,Hassinen IE

更新日期：1987-10-01 00:00:00

abstract：:Insulin-like growth factor-I (IGF-I) (13 nM) can replace insulin (0.8 microM) in a serum-free medium containing epidermal growth factor (EGF) (16 nM) and dexamethasone (DEX) (140 nM) and stimulate DNA synthesis in young cultures of WI-38 cells, similar to the stimulation of serum-supplemented medium. By contrast, sene...

journal_title：Journal of cellular physiology

authors： Phillips PD,Pignolo RJ,Cristofalo VJ

更新日期：1987-10-01 00:00:00

abstract：:We report here that heptanol (3.5 mM) induces in vitro a rapid formation of smooth endoplasmic reticulum aggregates (SERA) within isolated islets of Langerhans. SERA appeared after only 15 min of exposure to the alkanol and increased in number during the first 30 min of incubation. At that time, SERA represented 2% an...

journal_title：Journal of cellular physiology

authors： Knodel S,Meda P,Orci L

更新日期：1987-10-01 00:00:00

abstract：:The role of the plasma membrane potential (delta psi p) in the commitment to differentiation of murine erythroleukemia (MEL) cells has been studied by analyzing the ionic basis and the time course of this potential in the absence or the presence of different types of inducers. delta psi p was determined by measuring t...

journal_title：Journal of cellular physiology

authors： Arcangeli A,Ricupero L,Olivotto M

更新日期：1987-09-01 00:00:00

abstract：:Five-week-old female mice supplemented with estradiol and progesterone are able to respond to epidermal growth factor (EGF) and EGF-like growth factors (alpha-transforming growth factor [alpha-TGF] and crude mammary-derived growth factor) with local lobuloalveolar development when these growth factors are directly int...

journal_title：Journal of cellular physiology

authors： Vonderhaar BK

更新日期：1987-09-01 00:00:00